Oligonucleotide microarray has emerged as a powerful tool to efficiently address various problems in molecular biology and medical sciences [1–3]. The technique allows simultaneous detection of many different targets in a sample based on base-pairing rules.
The technique allows simultaneous detection of many different targets in a sample based on base-pairing rules. The most commonly used approach for construction of oligonucleotide-based microarrays, the deposition method, involves the covalent immobilization of amino- or mercaptoalkyl probes on modified glass surface [4–6]. Of these, mercaptoalkylated probes
have been immobilized on polymer surface carrying a variety of thiol group-specific functionalities, namely iodo-, bromo-, and maleimidoalkyl.